Copper and cadmium increase laccase activity in Pleurotus ostreatus
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چکیده
منابع مشابه
Copper induction of enhanced green fluorescent protein expression in Pleurotus ostreatus driven by laccase poxa1b promoter.
In silico analyses of several laccase promoter sequences have shown the presence of many different responsive elements differentially distributed along the promoter sequences. Analysis of Pleurotus ostreatus laccase promoter poxa1b extending around 1400-bp upstream of the start codon showed the presence of several putative response elements, such as 10 metal-responsive elements. Development of ...
متن کاملCopper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus.
Pleurotus ostreatus is a white rot basidiomycete that produces several extracellular laccase isoenzymes, including phenol oxidase A1b (POXA1b), POXA2, and POXC. POXC was the most abundant isoenzyme produced under all of the growth conditions examined in this study. Copper was the most efficient inducer of laccase activity among the putative inducers tested. The amounts of all of the previously ...
متن کاملMetal-responsive elements in Pleurotus ostreatus laccase gene promoters.
Fungal laccase gene transcription is strongly induced by copper ions; notably, some laccase promoters contain multiple putative metal-responsive elements (MREs). Previously, it has been demonstrated that the Pleurotus ostreatus laccase genes poxc and poxa1b are transcriptionally induced by copper, and several putative MREs were found in the promoter regions of these genes, which extend for abou...
متن کاملHeterologous Expression of Laccase (LACP83) of Pleurotus ostreatus
The heterologous expression of the gene LACP83 (encoding a laccase) from Pleurotus ostreatus in Escherichia coli was characterized. The laccase enzyme activity and kinetics of bacterial growth with an inducer (IPTG) and without inducer were determined. The maximum enzymatic activity was observed at 7 h post induction with a value of 3740 ± 342 U/L, which was similar to that reported for the nat...
متن کاملCharacterization of the Growth and Laccase Activity of Strains of Pleurotus Ostreatus in Submerged Fermentation
Kinetic parameters of growth and laccase activity of five ATCC strains of Pleurotus ostreatus in submerged fermentation were evaluated. The best strain for laccase production and the time of maximum laccase activity were also determined. The greatest laccase activity (37490 U/L), laccase productivity (78 U/L h), specific growth rate (0.026/h), and specific rate of laccase production (119 U/gX h...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2002
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(01)00519-5